The investment in Science Creates, backed by Research England, part of UKRI, and the University of Bristol, will see a 30,000 sq ft building provide capacity to incubate approximately 275 new companies from West of England universities and beyond.
Specifically, it will accommodate spin-outs which are making ground-breaking discoveries in quantum and engineering biology.
The third incubator will increase its facilities by more than 65 per cent to 75,000 sq ft.
Harry Destecroix, founder of Ziylo and the Science Creates ecosystem and a former Insider 42 Under 42, said: "We founded Science Creates in 2015, just one year after spinning out Ziylo, because the biggest barrier we faced was access to advanced research facilities.
"We also wanted to find people who understood the unique challenges that deep tech spin-outs face. Being part of only 0.3 per cent of UK companies that are spinouts, we decided to do something about it."
He added: "UK spin-outs are, and will be, driving the fourth industrial revolution. All we want to do is help world-class engineers and scientists who have discovered important technological breakthroughs, to build impactful deep tech companies.
"This will improve the health of the human race and the health of our planet. The additional physical infrastructure will allow deep tech companies to scale and emerge."
Dr Jon Hunt, the University of Bristol's Executive Director for Research, Enterprise & Innovation, said: "The University of Bristol is a great powerhouse for spin-outs. This success is attributable to excellent research by world class academics combined with a high-quality research commercialisation team and a vibrant, entrepreneurial ecosystem.
"The new incubator, located in the heart of our new Temple Quarter Enterprise Campus, will help create the next generation of deep tech spin-outs, in quantum, cyber, engineering biology and much more."